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C1277-0518

Selectivity Comparison of Different C8 Bonded Phases for Tricyclic Antidepressants and Benzodiazepines

Columns             : ChraSil B100 C8 150mm x 4.6mm, 5 µm

Part Number       : C1515-B5032

Mobile Phase     : A: H2O + 0.1% HCOOH,

                            B: CH3CN + 0.1% HCOOH

Gradient             : 30% - 40% B in 15 mins, hold at 40% B for 15 mins

Flow Rate            : 1.0 mL/min

Temperature        : Ambient

Detection            : UV, 254 nm

Peak Identification

1. 7-Aminoclonazepam

2. 7-Aminoflunitrazepam

3. Nordoxepin

4. Doxepin

5. Desipramine

6. Imipramine

7. Nortriptyline

8. Amitriptyline

9. Trimipramine

10. Clomipramine

11. Nordiazepam

12. Clonazepam

13. Flunitrazepam

14. Diazepam

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ChraNotes at https://chranote.com/C1277-0518