# C2084-0506 Determination of Unsaturated Fatty Acids via LC
Column : ChraSil D120 Phenyl, 150mm x 4.6mm, 5µm Part No. : [C1448-D5032](https://chrasil.com/C1448-D5032 "C1448-D5032") Mobile Phase : A: H2O + 0.1% HCOOH, B: CH3CN + 0.1% HCOOH A:B - 20:80 Flow Rate : 1.5 mL/min Temperature : Ambient Detection : UV, 214 nm **Peak Identification** 1. Linolenic acid 2. Linoleic acid 3. Oleic acid
[![image-1627382032831.png](https://chratec.info/uploads/images/gallery/2021-07/scaled-1680-/image-1627382032831.png)](https://chratec.info/uploads/images/gallery/2021-07/image-1627382032831.png) ChraNotes