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C2087-0509

Determination of Caffeine Metabolites by HPLC-UV together with ChraSil B100 C8, 150mm x 4.6mm, 5µm 

Column             : ChraSil B100 C8, 150mm x 4.6mm, 5µm

Part No.            : C1515-B5032

Mobile Phase   : CH3OH:1% CH3COOH in H2O - 10:90

Flow Rate          : 1.0 mL/min

Temperature     : Ambient

Detection          : UV, 254 nm

Peak Identification

  1. 7-Methylxanthine
  2. 1-Methyluric acid
  3. 3-Methylxanthine
  4. 1-Methylxanthine
  5. 3,7-Dimethylxanthine
  6. 1,7-Dimethylxanthine
  7. 1,3-Dimethylxanthine

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 ChraNotes