C2087-0509 Determination of Caffeine Metabolites by HPLC-UV together with ChraSil B100 C8, 150mm x 4.6mm, 5µm  Column             : ChraSil B100 C8, 150mm x 4.6mm, 5µm Part No.            : C1515-B5032 Mobile Phase   : CH 3 OH:1% CH 3 COOH in H 2 O - 10:90 Flow Rate          : 1.0 mL/min Temperature     : Ambient Detection          : UV, 254 nm Peak Identification 7-Methylxanthine 1-Methyluric acid 3-Methylxanthine 1-Methylxanthine 3,7-Dimethylxanthine 1,7-Dimethylxanthine 1,3-Dimethylxanthine  ChraNotes